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Of note, the same Ch AT-IRES-Cre knock-in allele is also available as C57BL/6N-congenic (Stock No. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Cre recombinase activity is reported in all cholinergic neurons.

These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research. A targeting vector was designed to insert an optimized internal ribosome entry site-linked Cre recombinase gene (followed by an frt-flanked neomycin resistance cassette) downstream of the stop codon of the endogenous gene.

Ch AT-IRES-Cre knock-in mice express Cre recombinase in cholinergic neurons, without disrupting endogenous Chat expression.

Give me a chance to talk through your questions about herpes, HPV or whatever is on your mind.”“I have been employed with ASHA for over 20 years and have been responding to calls about STIs, including herpes and HPV, for most of that time.Here are some potentially dangerous apps that are popular among kids: 1.Tinder: An app that is used for hooking-up and dating.Grindr is the world’s #1 FREE mobile social networking app for gay and bi guys to connect.Chat and meet sexy, attractive and interesting guys for free, or upgrade to Grindr XTRA for more features and more fun.Also, due to the rating system, it is often used for cyber-bullying, because a group of kids can target another kid and purposefully make his/her rating go down. Snapchat: This app allows a user to send photos and videos to anyone on his/her friend list.The sender can determine how long the receiver can view the image and then the image “destructs” after the allotted time.UPDATE: Get the 2015 Dangerous Apps List You may be thinking your kids are downloading apps because they are just a simple way for them to keep in contact with their friends.This is certainly true for most kids, but unfortunately, even innocent use of most of these apps can land a kid in a situation he/she never intended to be in.Problem: It is the #1 app used for sexting, mostly because people think it is the safer way to sext.However, the “snaps” can easily be recovered and the receiver can take a screen shot and share it with others.